DNA fingerprinting by arbitrarily primed PCR.
نویسندگان
چکیده
منابع مشابه
Molecular karyotype (amplotype) of metastatic colorectal cancer by unbiased arbitrarily primed PCR DNA fingerprinting.
Genomic instability characterizes the aneuploid cancer cell. Losses of genetic material are critical in cancer by exposing recessive mutations in tumor suppressor genes. Gains of genetic material also may lead to overexpression of genes contributing to tumor progression either in the presence or absence of mutation. However, the detection of moderate gains (such as tri-tetraploidy) has been a c...
متن کاملDNA fingerprinting of mammalian cell lines using nonradioactive arbitrarily primed PCR (AP-PCR).
probe. Retention of the probe on the dialysis bag using the electroelution protocol and inefficient binding of the probe to glass beads (as seen with ds DNA below 0.5 kb in size) contribute to probe losses. Since the ss probe does not need to be purified from the LMP agarose gel, loss of the probe associated with the two established protocols described here (particularly with the glass bead pro...
متن کاملRandomly Amplified DNA Fingerprinting: A Culmination of DNA Marker Technologies Based on Arbitrarily-Primed PCR Amplification
Arbitrarily-primed DNA markers can be very useful for genetic fingerprinting and for facilitating positional cloning of genes. This class of technologies is particularly important for less studied species, for which genome sequence information is generally not known. The technologies include Randomly Amplified Polymorphic DNA (RAPD), DNA Amplification Fingerprinting (DAF), and Amplified Fragmen...
متن کاملArbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica.
Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the cons...
متن کاملIncreased efficiency of arbitrarily primed PCR by prolonged ramp times.
for functional analysis. Mutagenesis to the small promoter-only containing plasmid is preferred to that of the reporter gene construct, because amplification of a smaller plasmid is faster and more efficient and removes the slight possibility of introducing random mutations to the reporter gene. The transferred mutant region can be sequenced to confirm that no random mutations were introduced d...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1994
ISSN: 1088-9051
DOI: 10.1101/gr.4.1.s59